Insertion junction: LMJ.RY0402.063479_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.063479
Chromosome:scaffold 36
Location:2271
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre36.g759597 (1 of 2) IPR001841//IPR013083//IPR024766 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, RING-H2-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACTGCTCATGTATGTCGAGCCACCATAG
Internal bar code:GTACGAGGGGTTGGAGGTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:95.1743
LEAP-Seq n confirming:1420
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTACGATTTGCGTGGGAC
Suggested primer 2:AACATCGTAGGAGGCCACAG