Insertion junction: LMJ.RY0402.063479_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063479
Chromosome:scaffold 36
Location:2353
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre36.g759597 (1 of 2) IPR001841//IPR013083//IPR024766 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, RING-H2-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTCCATGCTGACGGACCGCATCCAGCTC
Internal bar code:GCCTGAAGGATGTGGCGCAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:99.4914
LEAP-Seq n confirming:14279
LEAP-Seq n nonconfirming:73
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTACGATTTGCGTGGGAC
Suggested primer 2:AACATCGTAGGAGGCCACAG