| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063481 |
| Chromosome: | chromosome 6 |
| Location: | 5049872 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280450 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCATCATTATACACACGGATGCAGGCA |
| Internal bar code: | GGAATTCAAGTGGCCACCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.5522 |
| LEAP-Seq n confirming: | 3335 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTATTCCATGAAACGGCA |
| Suggested primer 2: | GTTACGGCGTGAGCTAGAGG |