| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063532 |
| Chromosome: | chromosome 2 |
| Location: | 804089 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078885 | (1 of 2) PTHR10997//PTHR10997:SF7 - IMPORTIN-7, 8, 11 // IMPORTIN-11 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATAAGCTCACTCGCGCCCCTCATCTGAG |
| Internal bar code: | GCGCAGGACACAGGCGAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 297 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCAGCTACGACTACTTC |
| Suggested primer 2: | TGGGTCTCTCTGTGCTGTTG |