| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063544 |
| Chromosome: | chromosome_8 |
| Location: | 4930329 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g385250 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGTGGTGAAGCGGTTGTGTCTCATGTCC |
| Internal bar code: | TGAGTTTCTATTGACCGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 99.5887 |
| LEAP-Seq n confirming: | 1937 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTGGTGGACATCAGTG |
| Suggested primer 2: | GAGCTTCAGCTCAGCAAACC |