Insertion junction: LMJ.RY0402.063551_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063551
Chromosome:chromosome 2
Location:7367581
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g144950 KCN4 Voltage-gated K+ channel; (1 of 1) PF00027//PF07885 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion channel (Ion_trans_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCACAGCAGGGGCCTGTAGCAGGAGGGC
Internal bar code:CGTGGTGCTCCGGCTCCATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:278
LEAP-Seq percent confirming:99.3157
LEAP-Seq n confirming:1016
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACGCTCCTCCTCTTCAT
Suggested primer 2:AAGGTGTTCCACAGGTCCAG