| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063560 |
| Chromosome: | chromosome 16 |
| Location: | 1564185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653601 | MRPL33 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTCCTTTCTGGTCCCAACTCAGCATC |
| Internal bar code: | TTCACTATATCGGTGACGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 97.2632 |
| LEAP-Seq n confirming: | 4549 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAACACCTGTACATCCG |
| Suggested primer 2: | GAAGGGAGTGCGCTTGTTAG |