| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063575 |
| Chromosome: | chromosome 17 |
| Location: | 3524051 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725200 | (1 of 2) K05658 - ATP-binding cassette, subfamily B (MDR/TAP), member 1 (ABCB1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAAGCACAAACAGCTCCTGAGAGCGTGA |
| Internal bar code: | GTACTCGGCCAGTGCATAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 99.4005 |
| LEAP-Seq n confirming: | 1658 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCGAATGGTTGTGAAG |
| Suggested primer 2: | GCAATGAGGATGCAGACAGA |