| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063625 |
| Chromosome: | chromosome 12 |
| Location: | 5521876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531200 | FOX2 | Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCACGTGCCAGCGCACCTGTCGGTGCA |
| Internal bar code: | GCGACTGGGAACTTTATGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 98.5631 |
| LEAP-Seq n confirming: | 5213 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTAATCCCACAGCACCTC |
| Suggested primer 2: | GTGTAAGAGGGGTATCGGCA |