| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063631 |
| Chromosome: | chromosome 1 |
| Location: | 5767581 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041150 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATATGAACTTGTACCAAGCGCTCAAAA |
| Internal bar code: | GACCCTGGGCGTCGTGCGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 98.911 |
| LEAP-Seq n confirming: | 7448 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGACTCGGACATCACCTT |
| Suggested primer 2: | CCAACTCCACACGTCAACAC |