| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063633 |
| Chromosome: | chromosome_13 |
| Location: | 3741820 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589450 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGACTTACGCGGGAGATAAACGTACATA |
| Internal bar code: | AATGTTCCGTGTATGAATGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.4695 |
| LEAP-Seq n confirming: | 1500 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACGAAATTGTGAGCCC |
| Suggested primer 2: | GAGTGGCGCTACAGTGACAA |