| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063637 |
| Chromosome: | chromosome 11 |
| Location: | 1725914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467785 | (1 of 3) PTHR10625:SF102 - HISTONE DEACETYLASE 10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACCAGAACCCATAAGCCGGCATGTGA |
| Internal bar code: | ACCGGCAGGCCACAACATCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 99.4203 |
| LEAP-Seq n confirming: | 6346 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTACCGGTATGGTGGTG |
| Suggested primer 2: | AGGTATCTCATGGGCTGGTG |