| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063652 |
| Chromosome: | chromosome 13 |
| Location: | 1350704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571520 | ANK12 | Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGCTTGCTGTGCTTGCTGGCCGTGCA |
| Internal bar code: | TGTCTAAGGGCTGTCTGAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.4936 |
| LEAP-Seq n confirming: | 2161 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGGGGATAGATGTAGGG |
| Suggested primer 2: | CTGCATGCCTTACAAGACGA |