Insertion junction: LMJ.RY0402.063674_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063674
Chromosome:chromosome 5
Location:2817409
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g237450 PSRP1 Plastid-specific ribosomal protein 1; (1 of 1) PTHR33231//PTHR33231:SF1 - FAMILY NOT NAMED // 30S RIBOSOMAL PROTEIN-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGATCCAATCAGGGCTGACGCCCCTGCC
Internal bar code:CCGGCAATGGTGCGCTGTAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:892
LEAP-Seq percent confirming:99.5868
LEAP-Seq n confirming:4579
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAAATCTTGTGCAACAGC
Suggested primer 2:GTCTGAGCGTTGTGTGCTGT