Insertion junction: LMJ.RY0402.063676_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.063676
Chromosome:chromosome 12
Location:129148
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g484250 DHC1,IA1-DHC1alpha,IDA1,ROC34,PF9 (1 of 1) PTHR10676//PTHR10676:SF135 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 10, AXONEMAL; Inner Arm Dynein Heavy Chain 1/f-alpha CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAGGAACGGGAAGCCGTACTGGATGGAC
Internal bar code:CTTGGGGGCGGTCACGGGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:468
LEAP-Seq percent confirming:96.9849
LEAP-Seq n confirming:772
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACGAAGAACAGGATGGC
Suggested primer 2:CAGTTTGAGGAGAAGACGGC