| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063678 |
| Chromosome: | chromosome 12 |
| Location: | 5998640 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534961 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTGGACAGGCGTTGCAGCTCCGGGTAC |
| Internal bar code: | AAGCTAACCTGCTCCCGAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.0216 |
| LEAP-Seq n confirming: | 1923 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGTTGACACAATGAAA |
| Suggested primer 2: | CATATCAGCGTGGGTCCTCT |