| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063683 |
| Chromosome: | chromosome 3 |
| Location: | 8172827 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200350 | SMM12 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108//PTHR10108:SF706 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGACAGCCTTCTCGGCGAGTACGTGA |
| Internal bar code: | TGGCTCGCAGCGGTGAGTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 85.5911 |
| LEAP-Seq n confirming: | 6849 |
| LEAP-Seq n nonconfirming: | 1153 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGGTACGCTTCCAGAAT |
| Suggested primer 2: | CGGTAGTTAACCGCGAATGT |