Insertion junction: LMJ.RY0402.063684_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.063684
Chromosome:chromosome 1
Location:2803484
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g016570 (1 of 1) IPR000104//IPR000719//IPR001245//IPR002290//IPR011009//IPR016024//IPR020635 - Antifreeze protein, type I // Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Armadillo-type fold // Tyrosine-protein kinase, catalytic domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTATCACCCCCCTCGTCTTCTTCAACATG
Internal bar code:CAGATTCGACCCAGACAACTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:99.4161
LEAP-Seq n confirming:681
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACATGAAGACGTCGCT
Suggested primer 2:CCCACCTTCTGGTTGTCTGT