| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063691 |
| Chromosome: | chromosome 16 |
| Location: | 4806609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCATGAGCATGCAACCTGGGCTGGGATC |
| Internal bar code: | GCTCTGCGTGACGTGCATCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 74.4063 |
| LEAP-Seq n confirming: | 282 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGGGTGTGAAGAGATG |
| Suggested primer 2: | AATCGGGTCGCATAGCATAG |