| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063708 |
| Chromosome: | chromosome 16 |
| Location: | 1422234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652350 | CMA1,FAP183 | Chlamydomonas-specific membrane protein family A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAGGAAGAGTGAAGTGCGAAGCAGCGG |
| Internal bar code: | CTACCGCGGTGTTTGGATGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 88.7952 |
| LEAP-Seq n confirming: | 3368 |
| LEAP-Seq n nonconfirming: | 425 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCATGACCTCCTCACT |
| Suggested primer 2: | GTTCAGCTGCCTCATGACAA |