| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063711 |
| Chromosome: | chromosome 6 |
| Location: | 2271849 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266800 | (1 of 3) PTHR30535//PTHR30535:SF2 - VITAMIN B12-BINDING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACATACCGTTTGCCTACCTGCCAGCTG |
| Internal bar code: | GCTGCCTCCTTCTACCTGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1236 |
| LEAP-Seq percent confirming: | 99.6125 |
| LEAP-Seq n confirming: | 5913 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCGTGGTACTTGCGTG |
| Suggested primer 2: | CACAGCGTGATAGTGCTCGT |