Insertion junction: LMJ.RY0402.063711_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.063711
Chromosome:chromosome 6
Location:2272830
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGGCACTACACGCTCCACGTGTACGGTC
Internal bar code:GCTCAATCGATTGGCGAGCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:881
LEAP-Seq percent confirming:99.7031
LEAP-Seq n confirming:2015
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGACTCACAAACTCCGCA
Suggested primer 2:TGCAGTCGCTAGAGGTGTTG