| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063736 |
| Chromosome: | chromosome 1 |
| Location: | 5477142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038550 | SQD2 | Sulfoquinovosyldiacylglycerol synthase; (1 of 2) K06119 - sulfoquinovosyltransferase (SQD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTCCCCCGGCCGACGCGCGCGGGAGTT |
| Internal bar code: | TTGAGAGCAGCCAGCGGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 95.8893 |
| LEAP-Seq n confirming: | 1213 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGCTGGGCTTCGTAGTA |
| Suggested primer 2: | CTTCCAACGCCTCAGTTAGC |