Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.063740 |
Chromosome: | chromosome 1 |
Location: | 120914 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCTCGTGGTCTGTGCTTCACCCAATT |
Internal bar code: | GGGCAAGAACGATGTGAGTTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 838 |
LEAP-Seq percent confirming: | 97.9181 |
LEAP-Seq n confirming: | 5832 |
LEAP-Seq n nonconfirming: | 124 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTTTGCAACGCACCATAAT |
Suggested primer 2: | GTGGTCTTGCGTGTCCCTAT |