Insertion junction: LMJ.RY0402.063740_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.063740
Chromosome:chromosome 1
Location:120936
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGGAGACGTTTGTGTTCTGCCCAGTCTG
Internal bar code:GCGGTTTATAGTACACATGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:896
LEAP-Seq percent confirming:99.4073
LEAP-Seq n confirming:9393
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTGCAACGCACCATAAT
Suggested primer 2:GTGGTCTTGCGTGTCCCTAT