| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063756 |
| Chromosome: | chromosome 10 |
| Location: | 4705330 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTATCCGGTGTGATGGGTGTGAATGCGT |
| Internal bar code: | AAAGGAGTAAGGGACGCGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 99.7296 |
| LEAP-Seq n confirming: | 7008 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGCGTGTGGACGGATAC |
| Suggested primer 2: | CAGTATGACTCGGCGCTGTA |