| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063763 |
| Chromosome: | chromosome 9 |
| Location: | 5794443 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402108 | (1 of 1) PTHR13167//PTHR13167:SF25 - UNCHARACTERIZED // PIEZO, ISOFORM E; Piezo R-Ras-binding domain membrane protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCATCTCCCCCTGCCTGCTGCTGCAGC |
| Internal bar code: | ACGTGACGTGTCCGTTGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 25.8081 |
| LEAP-Seq n confirming: | 503 |
| LEAP-Seq n nonconfirming: | 1446 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCACACACACACACATTA |
| Suggested primer 2: | TAAAAGGGCTGTAGCAAGCG |