| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063773 |
| Chromosome: | chromosome 12 |
| Location: | 3440950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496400 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTACCTATTTTGCCGGCGCGCACTGT |
| Internal bar code: | AGGTACCTTCGAGGCGTAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.5012 |
| LEAP-Seq n confirming: | 4987 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAACAATACCACCAGCA |
| Suggested primer 2: | CATTGGATGCAGACCCTGTC |