Insertion junction: LMJ.RY0402.063789_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.063789
Chromosome:chromosome 4
Location:3396726
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g227500 SRR18 Scavenger receptor cysteine rich (SRCR) protein; (1 of 2) IPR001190//IPR006626//IPR011050//IPR017448 - SRCR domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // SRCR-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGTTGAACATTAGCGTAAGCGACGTCA
Internal bar code:TGTTATTTTAAACAAAGTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:294
LEAP-Seq percent confirming:16.9939
LEAP-Seq n confirming:277
LEAP-Seq n nonconfirming:1353
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACACACACACAC
Suggested primer 2:TCTGCTAGGGAGCCAAGTGT