Insertion junction: LMJ.RY0402.063793_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063793
Chromosome:chromosome 16
Location:1706936
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g654800 CPC2 Central pair microtubule complex 2; (1 of 1) PTHR24198//PTHR24198:SF25 - ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATTCGGTACGCAGGGAGACACGCTGCT
Internal bar code:GCTGCGACGGCAGGGGATGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:447
LEAP-Seq percent confirming:94.5175
LEAP-Seq n confirming:431
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCCAAACTAAACCGGACT
Suggested primer 2:AACTGCAACCCGTCAAAATC