| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063804 |
| Chromosome: | chromosome 9 |
| Location: | 3748876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391282 | XYT1 | (1 of 1) K03714 - glycoprotein 2-beta-D-xylosyltransferase (E2.4.2.38); Xylosyl transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGAAGCCCTTGGGCGCGCCGTCGAAGG |
| Internal bar code: | TGGTGAGCCCGCAGCTATGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.4619 |
| LEAP-Seq n confirming: | 2403 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTCATCTTGCCCTGGTT |
| Suggested primer 2: | ATGTTGAGCACATAGCGCAG |