Insertion junction: LMJ.RY0402.063807_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.063807
Chromosome:chromosome 16
Location:4874032
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g685900 PRM3,PRMT3 Protein arginine N-methyltransferase; (1 of 1) K11436 - protein arginine N-methyltransferase 3 [EC:2.1.1.-] (PRMT3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGTATGCTGCGCAGCACGCCACCCATA
Internal bar code:TTCTGCGATTCTCATAGGTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:321
LEAP-Seq percent confirming:96.7391
LEAP-Seq n confirming:178
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TATAGGACCCTCGCCTCCTT
Suggested primer 2:TTGAATGACAGACTGAGCGG