| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063827 |
| Chromosome: | chromosome 11 |
| Location: | 3663436 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482483 | (1 of 1) K12599 - antiviral helicase SKI2 (SKI2, SKIV2L) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTGTTCTGCTTCAGCAAGAAGAGGTG |
| Internal bar code: | GCATTATGTAGTCTCCGCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 98.8095 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCCACACACTGCTCTC |
| Suggested primer 2: | CAACATCTCCACCACCTCCT |