| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063863 |
| Chromosome: | chromosome 10 |
| Location: | 595323 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421750 | CGL32,FAX1 | (1 of 2) PTHR12668:SF0 - UPF0136 MEMBRANE PROTEIN CG5532; Fatty Acid Export protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATTACTGCGTATAGACAAAGCGGGTTG |
| Internal bar code: | TTCACCCAGGTCATTCGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.8384 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGAGGGGTCACAGGTT |
| Suggested primer 2: | GAAGAATGCGTAGCGGAGAC |