| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063906 |
| Chromosome: | chromosome 9 |
| Location: | 498140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404201 | PHC30,PHC24,PHC10 | Pherophorin-chlamydomonas homolog 30; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACCCCTCTTCTGTCCAAGCAACGCGC |
| Internal bar code: | TCCGGGCAACGGCAGTCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 91.7644 |
| LEAP-Seq n confirming: | 3365 |
| LEAP-Seq n nonconfirming: | 302 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGGACTGGCACAGACT |
| Suggested primer 2: | ACCCGAAACAACTCGAACAC |