| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063962 |
| Chromosome: | chromosome 3 |
| Location: | 4456425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175851 | (1 of 13) IPR001487 - Bromodomain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTTTGAGAAGGTCGTCACGCTGGTGT |
| Internal bar code: | TTGCGGTAGTCTAATGAGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 99.2534 |
| LEAP-Seq n confirming: | 4653 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGTTACACCGCCACCCAT |
| Suggested primer 2: | TGGAGTCATTTGCTCACAGC |