| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063990 |
| Chromosome: | chromosome 14 |
| Location: | 571310 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611750 | (1 of 24) IPR001680//IPR017986//IPR020472 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCCGTGGGCAACGCCTCTACGGCGTC |
| Internal bar code: | GAGTATTTGGGCGGTGCGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 90.8896 |
| LEAP-Seq n confirming: | 8041 |
| LEAP-Seq n nonconfirming: | 806 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGCACTGGTCCGACTAC |
| Suggested primer 2: | GCCTGTGACTTCCTTCTTGC |