Insertion junction: LMJ.RY0402.064001_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064001
Chromosome:chromosome 16
Location:1081013
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g649550 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCCGCGCGGCCCTGCTGGCAACCCAC
Internal bar code:AATGATTGGCGAAGAGAGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:771
LEAP-Seq percent confirming:98.4264
LEAP-Seq n confirming:8194
LEAP-Seq n nonconfirming:131
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CTACGCCGACCTGTCTCTTC
Suggested primer 2:TGTTATTGCTGCTACTGCCG