| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064007 |
| Chromosome: | chromosome 1 |
| Location: | 2996825 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018400 | ITPK2,IPK | (1 of 2) K00913 - inositol-1,3,4-trisphosphate 5/6-kinase / inositol-tetrakisphosphate 1-kinase (ITPK1); Inositol 1%252C3%252C4-trisphosphate 5/6-kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTTTATGGACTATCGCATCGTATTCCC |
| Internal bar code: | AAGCCCCCTGAAGCGCCGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.5472 |
| LEAP-Seq n confirming: | 14070 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGACACAAACCATCGTCG |
| Suggested primer 2: | GACATGCCGAACACAATACG |