| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064008 |
| Chromosome: | chromosome 13 |
| Location: | 4165572 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592050 | DAL1 | Allantoinase; (1 of 1) 3.5.2.5 - Allantoinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATTGGTGACGGCCTGGTGCACAGCCCT |
| Internal bar code: | TACTTGACGATGGCCGACGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.5913 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGCTCGCCATCACAAGT |
| Suggested primer 2: | CTGTCCTGTTCTCCTCAGCC |