Insertion junction: LMJ.RY0402.064109_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.064109
Chromosome:chromosome 3
Location:8244900
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g199550 (1 of 1) IPR000504//IPR012677//IPR014720 - RNA recognition motif domain // Nucleotide-binding alpha-beta plait domain // Double-stranded RNA-binding domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGCGTTGGCGACGCGACTACTAGCACC
Internal bar code:AGACCAGGAAAGGCTCGTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:144
LEAP-Seq percent confirming:76.0148
LEAP-Seq n confirming:206
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACCCTCCCAACCTTACA
Suggested primer 2:CGAACAGTCCTTGTCCCTGT