| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.064133 |
| Chromosome: | chromosome 4 |
| Location: | 689169 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217953 | (1 of 2) PTHR15241:SF0 - TRANSFORMER-2 SEX-DETERMINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCAGTGCTGGACACGGCCCTCAGCCAC |
| Internal bar code: | TGAGAGCAGGCGGGCGGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 99.6736 |
| LEAP-Seq n confirming: | 1527 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTTTGCTGAAGCCACTA |
| Suggested primer 2: | ATGGTCTGCTGAGGATGCTT |