Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.064178 |
Chromosome: | scaffold 18 |
Location: | 120676 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre18.g748997 | (1 of 3) PTHR11132//PTHR11132:SF64 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCAGGCGATGCCCTCCGGCGAGGCGT |
Internal bar code: | GAGGTAGTGGTGGAGACGTCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 606 |
LEAP-Seq percent confirming: | 92.7681 |
LEAP-Seq n confirming: | 372 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCCAACAGGTACGCCTTC |
Suggested primer 2: | TACTGTAGGCCTCCACACCC |