| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064181 |
| Chromosome: | chromosome 6 |
| Location: | 3885795 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278178 | (1 of 1) PF09495 - Protein of unknown function (DUF2462) (DUF2462) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGCAACGAGGGCAGCTTGGCAGGTGCG |
| Internal bar code: | TGCGGGTCCACGATACGAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 93.9554 |
| LEAP-Seq n confirming: | 5689 |
| LEAP-Seq n nonconfirming: | 366 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAACAAAACCCTCAAAAT |
| Suggested primer 2: | GGCAGACAGTCAGCAAATGA |