| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064196 |
| Chromosome: | chromosome 1 |
| Location: | 4442275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030150 | (1 of 1) PTHR13489:SF0 - MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTCCCGCCCCCGGTTCACTGCAACAGC |
| Internal bar code: | GGGAAGGCCGCTGGTCAAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.5796 |
| LEAP-Seq n confirming: | 4974 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTTCCTAGCCTCTCGG |
| Suggested primer 2: | ACATTCACATCGAACCCCAT |