| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064226 |
| Chromosome: | chromosome 4 |
| Location: | 2287020 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220350 | ATPVA3 | Vacuolar ATP synthase subunit A; (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCGACCTGCAGCGAACACGACTGCTGC |
| Internal bar code: | ACGTTGTACGCTTACACATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.5982 |
| LEAP-Seq n confirming: | 6197 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGATTGGGAGATTGGGA |
| Suggested primer 2: | GAGCATTCTCAAAAGCCCTG |