| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064227 |
| Chromosome: | chromosome_3 |
| Location: | 5077021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g181150 | DLL1,ROC106,FLA14,RSP22,LC8 | Dynein arm light chain 8, LC8 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTACTCACTCCACACCTAACTACTAGCAAA |
| Internal bar code: | GCATTTAGACCCTCTGGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.7059 |
| LEAP-Seq n confirming: | 339 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGTATTTCGACCCTGCG |
| Suggested primer 2: | CATCTTCCTCCCCTTCCTTC |