Insertion junction: LMJ.RY0402.064231_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064231
Chromosome:chromosome 2
Location:6737740
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g118350 MFT14 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCTGCGTTACCCCTTCGGCCCTTCCCAA
Internal bar code:GGGGGATGCCTGGAAACGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:851
LEAP-Seq percent confirming:99.8255
LEAP-Seq n confirming:572
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TATTAGAGACAACGCCGCCT
Suggested primer 2:TTGGTCGTATTATGGGGGAA