| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064240 |
| Chromosome: | chromosome 11 |
| Location: | 411729 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467582 | RIB21,FAP306 | Ribbon binding 21 kDa microtuble inner protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCTGTGGATGCAGCGGGTGTGTCGCCCT |
| Internal bar code: | TGCTTGTGCATACGTTCAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.7568 |
| LEAP-Seq n confirming: | 16000 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCATTACTTGTTGGCGT |
| Suggested primer 2: | AAAGCGTGTATGTTCCCAGG |