| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.064246 |
| Chromosome: | chromosome 17 |
| Location: | 4250425 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730550 | (1 of 3) PTHR22298:SF39 - ENDOGLUCANASE 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATAGCTAGGACAACGGGCAGCAGCCG |
| Internal bar code: | GTTTCGCATACCTTTTTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.8783 |
| LEAP-Seq n confirming: | 821 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTTAGGATGCTGCTGGC |
| Suggested primer 2: | GATCGCTTAGCACAGGGAAG |